Development and Characterization of EST-SSR Markers for Ottelia acuminata var. jingxiensis (Hydrocharitaceae)

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Development and characterization of EST-SSR markers for Ottelia acuminata var. jingxiensis (Hydrocharitaceae)1

Premise of the study Simple sequence repeat (SSR) markers were derived from transcriptomic data for Ottelia acuminata (Hydrocharitaceae), a species comprising five endemic and highly endangered varieties in China. Methods and Results Sixteen novel SSR markers were developed for O. acuminata var. jingxiensis. One to eight alleles per locus were found, with a mean of 2.896. The observed and exp...

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Genetic diversity of Ottelia acuminata ( Hydrocharitaceae ) from the Eastern Himalayas , revealed by ISSR markers

Ottelia acuminata (Gagnep.) Dandy (Hydrocharitaceae), an endangered aquatic species, was investigated in the Eastern Himalayas, especially in Yunnan Province of Southwest China. The genetic diversity among seven populations was examined using inter-simple sequence repeat (ISSR) amplification markers. The field survey showed that 43.5% natural populations of O. acuminata have become extinct duri...

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Development and characterization of 25 EST-SSR markers in Pinus sylvestris var. mongolica (Pinaceae)1

UNLABELLED PREMISE OF THE STUDY A set of novel expressed sequence tag (EST) microsatellite markers was developed in Pinus sylvestris var. mongolica to promote further genetic studies in this species. • METHODS AND RESULTS One hundred seventy-five EST-simple sequence repeat (SSR) primers were designed and synthesized for 31,653 isotigs based on P. tabuliformis EST sequences. The primer pair...

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Development and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley

Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....

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Development and characterization of 29 polymorphic EST-SSR markers for Stipa purpurea (Poaceae)1

PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...

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ژورنال

عنوان ژورنال: Applications in Plant Sciences

سال: 2017

ISSN: 2168-0450

DOI: 10.3732/apps.1700083